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From three g of mobile paste about 100 mg of homogeneous protein were isolated. Enzymatic activity was dependent on the presence of disulfide minimizing reagents for example dithiothreitol or β-mercaptoethanol in the course of storage and purification. The native molecular body weight with the purified enzyme was estimated being one hundred eighty kDa dependent on the elution volume by way of a calibrated gel filtration column (data not proven).
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-acetyl-d-glucosamine-6-phosphate as illustrated in Scheme one. The deacetylation of this compound provides a supply of carbon and nitrogen by preparing this substrate for entry into the glycolytic pathway. This reaction is really a important action in the catabolism of N
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m with compound 1 is due to the protonation from the phosphate moiety to some mono-anion. It is also curious to notice the ionization which is noticed at higher pH during the log k